Digital Holographic Microscopy (DHM) is the generation of computer images of a sample using holographic techniques.
A hologram results from the interference between the object wave reflected from a sample and magnified by a microscope objective, and a reference wave.
Using a laser source, the small angle between the waves exhibits fringes that carry the phase and amplitude information in a single image - the hologram which is captured on a digital camera in a few microseconds.
The captured image is transmitted to a computer where numerical procedures are applied to reconstruct a 3D image of the sample. This process is called "image reconstruction".
The innovation of the DHM™ patented technology is the intervention of digital processing at a level that had not been reached so far in microscopy.